Whenever you run a precious sample and get different effects it can be as a result of tampering with the HPLC column in most cases. There are mixtures that need to be optimized to get the best results in a months’ time, it would not be right when you get results that cannot be interpreted it can be discouraging. There are high chances that you have a contaminated HPLC column. To avoid such a situation among others we are going to take you through troubleshooting tips that will help you know if you need to replace or recondition this HPLC column.

First you need to ensure if the column has been washed by the last user. Expert will tell you that after using all the time, the column needs to be stored in a miscible solvent for instance methanol. It is critical that you focus on using a solvent like methanol so that you can be able to enjoy the best services as this is one thing that has been featured mostly this time around and you need to take much care. It is essential that you note that when an organic solvent is added to the mobile phase, it will be effortless to re-equilibrate with the column.

At times the HPLC column, can have insoluble particles that may clog, you need to ensure that you take the right strategies to stay safe as this usually is very important. You need to carry out tests before you run your experiment through an external solubility or a dilution of the sample. There is need to know that for you to be able to have an easy time, ensure that you get to know how you can try more concentrated solvents to remove these parts as they have been seen to have a significant impact this time around. 

The extracts can also be removed by the use of the strong solvent that happens to be compatible with what you have been offering, it will help you know if this is what is needed for you. Take a moment and get to feature ways that you can be able to flush the insoluble materials using the backward form procedure, it can be delicate as it may result to destruction of the integrity of the bed and it matters much in the results that you get after separation.

If you do not take the issues in time, you may result to ruining all the results that you may be posting, you need to ensure that monitor your UHPLC column levels of pressures from time to time so that you can know if there are issues. You need to ensure that you boost performance all the time and if the troubleshooting does not bear any fruits, you need to ensure that you buy another HPLC column. For more information, click here: https://en.wikipedia.org/wiki/High-performance_liquid_chromatography.